electron ionization of serine and threonine: a discussion about peak intensities

Electron Ionization of Serine and Threonine: a Discussion about Peak Intensities

The present study describes the fragmentation under electron ionization (EI) of gas phase serine and threonine amino acids. Ab initio methods were performed to calculate the fragmentation paths and interpret the mass spectra. The six lowest energy conformers of L-serine, L-threonine and L-allo-threonine were obtained with B3LYP, G3MP2 and MP2 methods. The adiabatic and vertical ionizat...

Characterization of a Hank's type serine/threonine kinase and serine/threonine phosphoprotein phosphatase in Pseudomonas aeruginosa.

Pseudomonas aeruginosa is an opportunistic pathogen that causes infections in eye, urinary tract, burn, and immunocompromised patients. We have cloned and characterized a serine/threonine (Ser/Thr) kinase and its cognate phosphoprotein phosphatase. By using oligonucleotides from the conserved regions of Ser/Thr kinases of mycobacteria, an 800-bp probe was used to screen P. aeruginosa PAO1 genom...

Serine/threonine protein phosphatases.

1 (1-1) and inhibitor-2 (1-2), and preferentially dephosphorylate the /3-subunit of phosphorylase kinase, whereas type-2 phosphatases are insensitive to the heat-stable inhibitors and preferentially dephosphorylate the x-subunit of phosphorylase kinase [1,2]. Type-2 phosphatases can be further subdivided into spontaneously active (PP2A), Ca2l-dependent (PP2B) and Mg2+dependent (PP2C) classes. T...

Serine/Threonine Response Elements

T RANSCRIPTION in eukaryotes involves transcriptional activators, proteins that bind to specific sites distal from the TATA box termed enhancers or u p stream activation sequences (UASs) . When bound to a UAS, a transcriptional activator is able to stimulate the transcription initiation complex leading to synthesis of the mRNA. The ability of Saccharomyces cermisiar to use serine or threonine a...

Theoretical Thermodynamic Study of Solvent Effects on Serine and Threonine Amino Acids at Different Temperatures

The thermodynamic functions such as enthalpy (H), Gibbs free energy (G) and entropy (S) of Serineand Threonine amino acids were theoretically studied at different condition (solvents andtemperatures) by using Gussian o3, software. First, the structural optimization of isolated Serine andThreonine were done in the gas phase by using the Hartree-Fock (HF) level of theory with 3-21G, 6-31G and 6-3...

A serine/threonine phosphatase, PP2A, controls autoimmunity